AHD International, Importer and Distributor of Nutraceutical Ingredients

Anti-Oxidants & Immune Enhancing Ingredients

Cardiovascular Health

Ingredients for Healthy Joints and Cartilage

Memory & Mental Enhancing Ingredients

Menopause and Women's Health

Mood and Emotional Health

Weight Loss

All Natural Products

Product Infomation

Nutraceutical News
Clinical Studies



What's Hot?
Collagen Bulk Sales
Hoodia Bulk Sales
Hyaluronic Acid
Who We Are
About AHD
Contact AHD

Vitamin K2 Clinical Study

Stimulatory effect of menaquinone-7 (vitamin K2) on osteoblastic bone formation in vitro.

Yamaguchi M, Sugimoto E, Hachiya S.

Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.

Menaquinone-7, which is vitamin K2 (menatetrenone) with seven isoprene units, is highly contained in the fermented soybean. The effect of menaquinone-7 (MK-7) on osteoblastic bone formation was investigated. Femoral-diaphyseal and metaphyseal tissues of young male rats (4 weeks old) were cultured for 48 h in a medium containing either vehicle or MK-7 (10(-7)-10(-5) M). Calcium content, alkaline phosphatase activity, and deoxyribonuclic acid (DNA) content in the diaphyseal and metaphyseal tissues was significantly increased in the presence of MK-7 (10(-6) and 10(-5) M). The effect of MK-7 in increasing the diaphyseal and metaphyseal calcium content and alkaline phosphatase activity was completely prevented in the presence of cycloheximide (10(-6) M), an inhibitor of protein synthesis. Moreover, osteoblastic MC3T3-E1 cells after subculture were cultured for 24 h in a serum-free medium containing MK-7 (10(-7)-10(-5) M). Protein content, alkaline phophatase activity, osteocalcin and DNA content in the cells was significantly increased in the presence of MK-7 (10(-6) and 10(-5) M). The effect of MK-7 in increasing protein content, alkaline phosphatase activity, and osteocalcin production in the cells was completely blocked by cycloheximide. This study demonstrates that MK-7 has an anabolic effect on bone tissue and osteoblastic MC3T3-E1 cells in vitro, suggesting that the compound can stimulate osteoblastic bone formation.

PMID: 11681714 [PubMed - indexed for MEDLINE]

<< Back to Vitamin K2 Clinical Study List

We are strictly a bulk supplier to manufacturers – we do not sell to re-sellers or individuals. Our minimum order size is approximately 25 kilos. We are one of only a few suppliers that carry $5 million product liability insurance for our customers. AHD International LLC is classified as a importer, exporter, manufacturer, distributor of nutritional ingredients depending on the ingredient specified. AHD has 5 warehouse locations across North America and sales agents in over 10 countries. We are the only ingredient company to be awarded the Dun and Bradstreet's fastest growing company in America twice in 1999 and 2002.

For quotes please click here

*"These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease."

© AHD International, LLC.
Legal and Privacy Statement

Entrepeneur Hot 100

Named in Dun & Bradstreet Entrepreneur Magazine "Hot 100" fastest growing companies.
For Vendors

Get a Quote
Partner With Us

Choose AHD

AHD has over 650 customers.

5 North American warehouses making large quantity orders and quick delivery possible.

Representatives in USA, Mexico, Chile, Brazil and Uruguay.

AHD has a reputation for carrying the highest quality products in the industry.

Exclusivity of premium products from some of the top nutraceutical companies world. wide.


Industry News
Business News
Food & Beverage
Sports Nurtition